Rosario La Spina trained at the Queensland Conservatorium of Music continuing his studies at RNCM, Manchester under the tutelage of Joseph Ward OBE.  

Having gained first prize in the tenor competition held by L'Accademia di La Scala in 2000, Rosario embarked on the two-year Young Singers’ Course at La Scala, Milan, where he studied with Luciana Serra and Leyla Gencer.  This culminated in his La Scala debut as the Messenger in Samson and Delilah, where he appeared with Placido Domingo and Olga Borodina.  In 2002, Rosario won first prize in the Mario Del Monaco International Opera Competition and made his principal role debut at La Scala as Riccardo (Oberto) reprising the role in Genoa.

2003 saw his debut at the Verdi Festival at the Teatro Regio di Parma as Arvino (I Lombardi) – he also covered the role of Manrico (Il Trovatore) for Welsh National Opera and performed Alfredo (La Traviata) in Modena and Luigi (Il Tabarro) in Osaka, Japan.

In 2004, Rosario sang The Duke (Rigoletto) for New Zealand Opera, Cavaradossi (Tosca) under Richard Hickox at the Endellion Festival, Canio (Pagliacci) with the Taipei Symphony Orchestra, Taiwan and toured Spain in concert with the Parma Ensemble. He made his Opera Australia debut as Cavaradossi in 2005 and has subsequently appeared as The Prince (Love for Three Oranges), Alfredo, Pinkerton (Madama Butterfly), The Prince (Rusalka), Calaf (Turandot), Rodolfo (La bohème) and the title role of The Tales of Hoffmann. Other recent operatic engagements have included Alfredo for West Australian Opera and, in 2007, his American debut as Rodolfo for Seattle Opera.

Rosario has also been a regular guest soloist with the Sydney, Melbourne, Queensland and Adelaide Symphony Orchestras and appears on the CD/DVD Classical Spectacular with the MSO (ABC Classics).  In 2008, he sang Pinkerton in Melbourne, Don José (Carmen) in Sydney and Melbourne and returned to Seattle as Radames (Aida). In 2009, he performed Pinkerton in Sydney, Hoffmann in Adelaide, Radames in Sydney and Melbourne and Verdi’s Requiem in Brisbane and Adelaide.

Recent recordings include Puccini Romance with The Queensland Orchestra for ABC Classics, The Prince in Rusalka under Richard Hickox (Chandos) and, in 2009, a major solo album released by Universal Music featuring Rosario with the Orchestre Philharmonique de Monte Carlo. His 2010 Universal duo release Always You (with his sister, Anna-Maria La Spina) shot straight to the top of the classical charts in Australia.

Engagements for 2010 have included performing The Duke (Rigoletto) and Cavaradossi (Tosca) for Opera Australia; Radames (Aida) for Southern Opera, New Zealand, the Canadian Opera Company and the State Opera of South Australia. In 2011, he sings Pinkerton, Don José and Macduff (Macbeth) for Opera Australia, Hoffmann in Perth and Don José in Adelaide.